FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Skin and venereal diseases are among the oldest diseases of mankind that have accompanied humans at all stages of evolution. It is worth noting that the oldest example of medical literature, namely a clay tablet with a Sumerian text found in Nippur (in modern Iraq), tells about the treatment of skin diseases. After tens of thousands of years, I want to talk and write about skin and venereal diseases.
The assessment of the safety of products supplied to the market, without which the sale is impossible and illegal, protecting the consumer's health, does not guarantee him the effectiveness declared by the manufacturer. Meanwhile, there has long been equipment for assessing skin parameters, which in the hands of specialists can be used to objectively assess the effectiveness of cosmetic products and hardware methods.
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The history of dermatology remembers many attempts to transform human skin, a dense tissue, into a liquid. The search for a method to separate the skin's cellular substrate to obtain a suspension and study the phenotype of its constituent cells became the basis for a long-term research, resulting in a patented method for digitally assessing the subpopulation composition of skin cells - the skin cytoimmunogram... read the full article
