FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
As a doctor, I repeatedly observed the development of a human embryo from several cells, witnessing the mystery of the appearance of life in the embryological laboratory. Also, as when a long time ago an organism emerged from the first cells, and then a person developed, now having made a population of the planet in seven billion three hundred million, life on the Earth also originated. But, one thing is birth, and the other is development. Read more...
Recently I have been asked this question.
This has made me ponder. As you know only dead people do not perspire… If this question has been asked, if it concerns somebody including me and if my feelings give rise to new thoughts then he is rather alive. From the perspective of the doctor stating the facts of the patients being discontent of the quality of the medical care provided he may be dead!
But as the woman cannot be just a little bit pregnant then a person cannot be half or partly alive or dead. Besides ...
Проведен эксперимент на 40 половозрелых кроликах с целью сравнения пролиферативной и апоптотической активности клеток эпидермиса и дермы при формировании грубого соединительнотканного рубца и эпителизации раны без образования рубца с восстановлением функции поврежденного участка... читать о результатах эксперимента
Later we may ask ourselves where we are going and why. This thought came to my mind under the sign “CANNIBALISM POLICE”… But it was too late, we had to go farther consoling ourselves with the thought that we were among the very few people in the whole world who had made some steps forward from the sign. Read more...
