FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Проведен эксперимент на 40 половозрелых кроликах с целью сравнения пролиферативной и апоптотической активности клеток эпидермиса и дермы при формировании грубого соединительнотканного рубца и эпителизации раны без образования рубца с восстановлением функции поврежденного участка... читать о результатах эксперимента
Day in day, exactly 5 years, in Mikhail Sharomov's custom workshop, we created this unique custom motorcycle named Buffalo with Harley Davidson V-ROD engine. From idea and concept, design and implementation in every technical detail, through years of friendship, creation and inspired creativity to final assembly and registration. About that in my short film "Buffalo".
Достигнув очередного этапа по маршруту Перу-Боливия, экспедиция Живая Параллель, всем составом ощутила высоту 3200 метров. Лопнувшие сосуды глаз, необъяснимая усталость, повышенная утомляемость, головная боль, ломота в суставах плюс желание сжаться до размера рюкзачка, который висит на спине товарища и, спрятавшись в нём, отдаться размышлениям, а не борьбе с одышкой.
Quite early on, I realized that to truly help a large number of patients, I needed to go beyond the individual approach and create a methodology. I recall the words of Edith Piaf, who said, "I don't sing for everyone—I sing for everyone." And yet, she sang for everyone. So it was for me—there was that inspiration, that moment when the idea of phenotypic dermatology first arose. It was from this moment that the path to a systemic view of the skin began—not as a surface, but as an entire biological universe.
