FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Studied the proliferative potential and viability of hematopoietic stem cells (HSC) cord blood (PB), depending on the method of cryopreservation. Cryopreservation of samples was carried out in software Planer freezer and liquid nitrogen vapor. Found that, for cryopreservation of samples GSK PC software should be used freezer, since this method of preservation of the cells remain vi- able and functional completeness.
Представляю новый интегральный показатель — ФИВК (Фенотипический индекс воспаления кожи), предназначенный для количественной оценки воспалительных и репаративных процессов на клеточном уровне. Далее...
The creation of fertile innovative ecological environment in the country is constrained by barriers of a conceptual nature. The article identifies four groups of such barriers. The authors propose a strategy for overcoming them based on a comparison of functions necessary for the innovative development of social practices and the natural mechanisms of a new life birth. This allows us to formulate a number of pragmatic recommendations for creating an innovative eco-environment for a wide range of industries. By analogy with natural dynamics of obstetrics, the approach is called placental
Keywords: Innovation, innovation infrastructure, acceleration, eco-environment, placenta, development.
Оказаться в Лиме для всех участников экспедиции Живая Параллель – знаковое событие, именно здесь стартует исследование закономерностей культуры, о которой, согласитесь, у нас очень смутные представления. А иначе и быть не могло: инки, испанцы, индейцы и... метисы - много разнообразных культур и одна на всех Культура Перу, в которой нам предстоит побыть ближайшие недели.
