FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Рассмотрев различия в этиопатогенезе, клинической картине, а главное в подходах к терапии истинной и микробной экземы, позволю себе остановиться на одном интересном случае из практики, в качестве продолжения разговора об экземе. Этим постом хочу проявить уважение к представителю могущественной царственной семьи. Но, по-порядку. :)
Изобретение относится к области фармацевтики и биологии и представляет собой способ получения жизнеспособной гетерогенной популяции клеток кожи, включающий забор биоптата кожи на глубину ...
International Festival in Travel and Tourism named after N.N. Miklouho-Maclay "Russian traveler" was held in Orel.
My lecture "Diagnosis: The Traveler", planned in three parts, one for each day of the festival, was received with interest by world-famous travelers. But, as they say, tell God about your plans and he will laugh - and it happened. The third part, in my opinion the most important, because of the time-bound regulation was not brought to the listeners. However, in this there was a plus, as this is the best reason to continue the relationship outside the festival and breaking the boundaries, regardless of the distance to complete the scheduled. Let's do it, my friends!
Лима, словно узнав, что гости ненадолго, щедро одарила нас событиями дня и встречами, что оставляют следы, по которым наш разум путешествует в прошлом.
