FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Taking into account the universality of the pathogenesis of the wound process and the existence of substances predictably affecting the nature of the immune response in the skin and certain functions of its constituent cells (including reparative), the development of a new biotechnological agent for healing skin wounds of different etiology is justified and relevant.
This determined the aim and objectives of this study. Read more...
0
The creation of fertile innovative ecological environment in the country is constrained by barriers of a conceptual nature. The article identifies four groups of such barriers. The authors propose a strategy for overcoming them based on a comparison of functions necessary for the innovative development of social practices and the natural mechanisms of a new life birth. This allows us to formulate a number of pragmatic recommendations for creating an innovative eco-environment for a wide range of industries. By analogy with natural dynamics of obstetrics, the approach is called placental
Keywords: Innovation, innovation infrastructure, acceleration, eco-environment, placenta, development.
0
Казалось бы, что может быть общего между коренными народами Крайнего Севера и африканцами? При всем желании очень трудно найти сходство между ненцами, хантами и представителями племен датогов и масаи, проживающих в Танзании. С таким же успехом можно пытаться сравнить раскаленный африканский песок и жидкий азот, лед и пламя, жару и холод, черное и белое. Тем не менее, побывав во время недавней экспедиции в Африке, удалось увидеть то, что роднит народы, никогда не пересекавшиеся друг с другом.
0
Piogenic cocci, in the case of weakening the properties of the immune system of the skin (Yes, it is in the case of weakening the properties of immunity, because the normally functioning skin easily copes with numerous attacks of pyogenic microorganisms), can provoke the development of an island-inflammatory disease – pyoderma. Read more...
0
