FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Разобравшись в прошлый раз с механизмом этиопатогенеза, который уже на этом – раннем этапе понимания, определяет единые принципы лечения экземы независимо от её клинических разновидностей, на этот раз нам предстоит рассмотреть особенности развития хронического воспаления в дерме и эпидермисе, которые напротив, указывают на необходимость различий в подходе.
A good leader must combine several important qualities at once and perform many functions: competently manage the budget, be a mentor for subordinates and a friend to his clients, create a working atmosphere, set an example, solve problems, set tasks ... Simply put, to be the center of your business .
But there is one more duty that among others is usually not called: if the boss defaults, encourages and directs, he must also punish, and sometimes - severely. Of course, this is very few people enjoy, but it is - as an integral part of the leadership process, like the others.
About what misdemeanors of subordinates deserve indulgence, and which ones can not be forgiven in any case, and also what will be the most serious punishment for a doctor working in a medical institution, we talked with the "Aesthetic Guide".
Еду в такси и слышу песню из новогодней комедии про ироничность судьбы и лёгкий пар, на стихи Цветаевой: "Мне нравится, что вы больны не мной, мне нравится, что я больна не вами..." – сразу вспоминаю свои годы работы в областном кожно-венерологическом диспансере... читать об этом
Conversation on Facebook:
– Where are you?
– Travelling.
– Where this time?
– Where cannibals live.
– ...How are you?
– All parts of my body still belong to me!:)
Seems funny? Click the "like" button. Somebody will say – it can’t be true, what cannibals these days?! A year later after we spent 3 weeks in company with true cannibals as participants of the expedition "Live Parallel in Papua New Guinea", I can hardly believe it myself. Shall we remember it together?
