FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Few of my colleagues remember the glorious time when not only a comprehensive approach based on objective knowledge was used in the treatment of skin diseases and sexually transmitted infections, but also Immunostimulants were used, especially for the treatment of late forms of syphilis. Further...
The results of several patented inventions investigate the dynamics of human skin cell subpopulation states. The immunological phenomena discovered thereby change not only the picture of skin conditions, but also the ideas about approaches to the diagnosis and treatment of skin diseases... read the article in full
An unexpected interview came from a conversation with the journalist of the newspaper Tyumen News Irina Tarabaeva.
Определившись с механизмом развития клинических форм экземы и её этиопатогенезом, мы имеем все основания обсуждать различия в подходах к лечению. Они прослеживается в этиологическом и патогенетическом компонентах терапии, поскольку детерминированы разными первопричинами... читать далее
