FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
After twelve years of successful expeditionary experience, the LIVING PARALLEL broadens the horizon of its search. Since 2019, the program of continuous round-the-WORLD STUDY of the possibilities of Culture as a "second nature" and man in local cultures has been continuing.
Stage 1 has already taken place in New Zealand, and the next stage is ahead – South and Central Africa – 2021
The results of several patented inventions investigate the dynamics of human skin cell subpopulation states. The immunological phenomena discovered thereby change not only the picture of skin conditions, but also the ideas about approaches to the diagnosis and treatment of skin diseases... read the article in full
The assessment of the safety of products supplied to the market, without which the sale is impossible and illegal, protecting the consumer's health, does not guarantee him the effectiveness declared by the manufacturer. Meanwhile, there has long been equipment for assessing skin parameters, which in the hands of specialists can be used to objectively assess the effectiveness of cosmetic products and hardware methods.
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