FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Conversation on Facebook:
– Where are you?
– Travelling.
– Where this time?
– Where cannibals live.
– ...How are you?
– All parts of my body still belong to me!:)
Seems funny? Click the "like" button. Somebody will say – it can’t be true, what cannibals these days?! A year later after we spent 3 weeks in company with true cannibals as participants of the expedition "Live Parallel in Papua New Guinea", I can hardly believe it myself. Shall we remember it together?
Day in day, exactly 5 years, in Mikhail Sharomov's custom workshop, we created this unique custom motorcycle named Buffalo with Harley Davidson V-ROD engine. From idea and concept, design and implementation in every technical detail, through years of friendship, creation and inspired creativity to final assembly and registration. About that in my short film "Buffalo".
I don't remember any names. I don't know who these children have become. I don't know which of them is in school today, who is already working, who is raising younger brothers and sisters, and who has long been swept away by a road that we don't even know exists. But I remember their eyes.
Цельгель искусственно повышает концентрацию сигнальных молекул в ране, чтобы активировать механизмы репарации и ускорить естественные механизмы регенерации кожи, которые у человека с возрастом ослабевают. Покупайте в клинику, покупайте себе...
