FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Conversation on Facebook:
– Where are you?
– Travelling.
– Where this time?
– Where cannibals live.
– ...How are you?
– All parts of my body still belong to me!:)
Seems funny? Click the "like" button. Somebody will say – it can’t be true, what cannibals these days?! A year later after we spent 3 weeks in company with true cannibals as participants of the expedition "Live Parallel in Papua New Guinea", I can hardly believe it myself. Shall we remember it together?
The Himba tribe is one of the most colorful and unique peoples of Africa, living in northern Namibia, in the Kunene region, near the border with Angola. It is a mistake to assume that these people are wild. Many Himba have dual citizenship - Himba and Namibia (not many of us can boast passports from two countries :)), but they do not fully recognize Western laws. Their culture, way of life and appearance have remained virtually unchanged for centuries despite globalization. It is understandable that young people leave for the cities, but they also often return to their roots. And why? Let's try to figure it out together...
Ещё одним важным отличием экспедиции является наличие вызова самим себе. Осознанное вопрошание, готовность встречи с явлениями, которые дадут ответ на вопросы или помогут разобраться с познавательными трудностями – всё это отразится результатами или, если хотите, итогами экспедиции. Без извлечения смыслов похода, путешествие превращается в бродяжничество.
