FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Surprisingly, there are 14 years between these books. That's exactly how long Leo Tolstoy felt one cycle of human maturation constitutes. He had an almost intimate idea of dividing life into periods—conditional periods in which, every fourteen years, a person reaches a new level of understanding.
If, putting aside excessive modesty (briefly and strictly for scientific purposes), we apply this logic to ourselves, a curious picture emerges.
The Himba tribe is one of the most colorful and unique peoples of Africa, living in northern Namibia, in the Kunene region, near the border with Angola. It is a mistake to assume that these people are wild. Many Himba have dual citizenship - Himba and Namibia (not many of us can boast passports from two countries :)), but they do not fully recognize Western laws. Their culture, way of life and appearance have remained virtually unchanged for centuries despite globalization. It is understandable that young people leave for the cities, but they also often return to their roots. And why? Let's try to figure it out together...
All my life, it seemed to me that answers make a person's thinking bigger and at the same time sharper. But over time, I realized that answers are born only where they are preceded by good questions. Not to the textbook or to the authority, but to reality itself — to what we see, but often do not notice. It is often said that human life is short. I am becoming more and more convinced that it can be "lengthened" — not by the number of years I have lived, but by the density of observations. In more detail...
or immediately into the telegram channel from observation to understanding
The invention relates to the field of pharmaceuticals and biology and represents a method for obtaining a viable heterogeneous population of skin cells, which includes taking a skin biopsy at a depth of ...
