FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
The history of dermatology remembers many attempts to transform human skin, a dense tissue, into a liquid. The search for a method to separate the skin's cellular substrate to obtain a suspension and study the phenotype of its constituent cells became the basis for a long-term research, resulting in a patented method for digitally assessing the subpopulation composition of skin cells - the skin cytoimmunogram... read the full article
Information about the history of medical institutions Tyumen, which used to belong to the Tobolsk province, has been preserved a little. It is known that in 1741 there was a hospital in the city, which had 3 attendants. The hospital was located at the monastery. In the first half of the 19th century (1845), there was already one hospital in the city. There were 5 officials (doctors) and 7 lower attendants (orderlies) in the medical department. In 1859, the city hospital was opened, considered by contemporaries to be the best in Western Europe. Siberia, which has six hospital rooms, a laboratory, a pharmacy, and an anatomical theater... The whole story
В прежние времена, будь то в СССР или Англии, чтобы позвонить из автомата на улице, мы платили денежку и несколько минут у тебя было для разговора. Куда подевалось это прекрасное условие? есть предложение
The article makes it clear that the problems of public health service are strategic in nature. That is why only tactical strategic decisions on its development can significantly influence such basic indicators as the sickness rate, the life-span and the ability to work of the population. The conceptual nature of public health service, substantiating the necessity of its conceptual restructuring, is revealed. The scenario of public health service innovative upgrade based on the conceptual technologies, taking into account of the system approach and the cultural context in their inseparability is suggested.
Keywords: Public health care, conceptual restructuring, innovative scenario, system approach, the cultural context
