FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
I want to take a breath – we have done it! The expedition Live Parallel 2013 – Papua New Guinea where we walked many kilometers through the jungle, spent tens of kilometers inside the tarantas and hundreds of kilometers on board small planes flying on the same principle as a route-taxi, has come to an end without loss and above all with the result! But it could be otherwise… Well, let us not talk about sad things but about interesting ones, so… Jakarta was the first place to see.
Пятнадцать лет NEO-Clinic. 15 лет бизнесу, компании, имени. Но, если для человека – это финишная прямая на пути к совершеннолетию, то для современного медицинского учреждения – это время зрелости. А зрелость в свою очередь определяется как пик возможностей... о традициях, развитии и миссии NEO-Clinic
Parallel worlds exist not only in the books by science fiction writers. One can find them in medicine, too. In this article I will share the analogies that I saw visiting the production facility Lumenis in Israel. It turned out that we have a lot in common with the Israel developers. So ...
