FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Оценка безопасности поставляемой в рынок продукции, без которой реализация невозможна и незаконна, охраняя здоровье потребителя, не гарантирует ему заявленную производителем эффективность. Между тем, давно существует оборудование для оценки параметров кожи, которое в руках специалистов может быть использовано для объективной оценки эффективности косметической продукции и аппаратных методов.
A good leader must combine several important qualities at once and perform many functions: competently manage the budget, be a mentor for subordinates and a friend to his clients, create a working atmosphere, set an example, solve problems, set tasks ... Simply put, to be the center of your business .
But there is one more duty that among others is usually not called: if the boss defaults, encourages and directs, he must also punish, and sometimes - severely. Of course, this is very few people enjoy, but it is - as an integral part of the leadership process, like the others.
About what misdemeanors of subordinates deserve indulgence, and which ones can not be forgiven in any case, and also what will be the most serious punishment for a doctor working in a medical institution, we talked with the "Aesthetic Guide".
As a doctor, I repeatedly observed the development of a human embryo from several cells, witnessing the mystery of the appearance of life in the embryological laboratory. Also, as when a long time ago an organism emerged from the first cells, and then a person developed, now having made a population of the planet in seven billion three hundred million, life on the Earth also originated. But, one thing is birth, and the other is development. Read more...
Studied the proliferative potential and viability of hematopoietic stem cells (HSC) cord blood (PB), depending on the method of cryopreservation. Cryopreservation of samples was carried out in software Planer freezer and liquid nitrogen vapor. Found that, for cryopreservation of samples GSK PC software should be used freezer, since this method of preservation of the cells remain vi- able and functional completeness.
