FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Later we may ask ourselves where we are going and why. This thought came to my mind under the sign “CANNIBALISM POLICE”… But it was too late, we had to go farther consoling ourselves with the thought that we were among the very few people in the whole world who had made some steps forward from the sign. Read more...
I am aware, colleagues, that here we "treat" metaphysically, and not a specific patient, so the text sins universality, but - as a guide - will help us to treat consciously, in accordance with the stages of inflammation and focusing on the observed phenomena. Read more...
Information about the history of medical institutions Tyumen, which used to belong to the Tobolsk province, has been preserved a little. It is known that in 1741 there was a hospital in the city, which had 3 attendants. The hospital was located at the monastery. In the first half of the 19th century (1845), there was already one hospital in the city. There were 5 officials (doctors) and 7 lower attendants (orderlies) in the medical department. In 1859, the city hospital was opened, considered by contemporaries to be the best in Western Europe. Siberia, which has six hospital rooms, a laboratory, a pharmacy, and an anatomical theater... The whole story
