My projects
Photo Video Blog Books
Goltsov Sergey
I serve the art of medical doubt

METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM

FIELD: medicine.

SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.

EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.

 

Ещё в блоге:

Wound-healing Cellgel
Wound-healing Cellgel

The ability to repair is the most important property of the skin that determines the closure of a wound or injury "at all costs". However, healing can be with or without scarring. So what is the secret?

 

 

Rosacea, we treat consciously

I am aware, colleagues, that here we "treat" metaphysically, and not a specific patient, so the text sins universality, but - as a guide - will help us to treat consciously, in accordance with the stages of inflammation and focusing on the observed phenomena. Read more...

Натурально как вы играете. И царь у вас такой… типичный!
Натурально как вы играете. И царь у вас такой… типичный!

Рассмотрев различия в этиопатогенезе, клинической картине, а главное в подходах к терапии истинной и микробной экземы, позволю себе остановиться на одном интересном случае из практики, в качестве продолжения разговора об экземе. Этим постом хочу проявить уважение к представителю могущественной царственной семьи. Но, по-порядку. :)

The journey, like a little life or somebody to take on the job

Newspaper Tyumen news, in the face of Irina Tarabaeva, again I was able to talk...

Read more...