FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
– Honey, what can I bring you from the expedition?
– You may bring what they want, now all treated...
This short dialogue with my wife, colleagues, I want to go to the site the Jungle of dermatology to find a place for an integral part of our specialty, which always hides in the disguise of garments, and social twists and turns of life, manifesting itself unexpectedly, and event and frighteningly, like a bird taking off from the thick foliage. Her name is venereology.
В любой культуре есть ритуалы. Некоторые направлены на укрепление здоровья — и физического, и психического. Об одном из таких ритуалов я хочу рассказать.
Экспедиция Живая Параллель, как её первая часть – Science в Перу, так и вторая – Experience в Боливии, закончились.
As a doctor, I repeatedly observed the development of a human embryo from several cells, witnessing the mystery of the appearance of life in the embryological laboratory. Also, as when a long time ago an organism emerged from the first cells, and then a person developed, now having made a population of the planet in seven billion three hundred million, life on the Earth also originated. But, one thing is birth, and the other is development. Read more...
