FIELD: medicine.
SUBSTANCE: method for determining the subpopulation composition of skin cells and obtaining the skin cytoimmunogram comprises sampling a skin biopsy specimen to a depth of 2 mm, homogenizing the tissue in a 0.9% aqueous solution of sodium chloride at a temperature of +23…+25°C, extracting the homogenate, filtering the homogenate through an inert filtering partition with a pore diametre of 20 mcm, centrifuging the homogenate at 400 g for 5 minutes at a temperature +23…+25°C. After centrifugation, the viability of the skin cells is determined, the skin cells are incubated for 20 minutes with monoclonal antibodies fluorochrome-labeled for flow cytometry, phenotyping of the skin cells is carried out, the number of skin cells of a specific phenotype - skin cytoimmunogram - are determined: keratinocytes CD49f+, among them activated CD49f+HLA-DR+; fibroblasts (fibrocytes) CD45-CD14-CD44+, among them activated CD45-CD14-CD44+CD80+; mast cells CD249+, among them activated CD249+CD63+; monocytes (macrophages) CD45+CD14+, among them activated CD45+CD14+HLA-DR+; intra-epidermal macrophages CD207+, among them activated CD207+CD80+, CD207+HLA-DR+, CD207+CD80+HLA-DR+; endothelial cells CD146+, among them activated CD146+CD34+, CD146+HLA-DR+, CD146+CD54+, CD146+CD54+HLA-DR+; lymphocyte populations: T-lymphocytes CD45+CD3+, T-helpers CD45+CD3+CD4+CD8-, T-suppressors CD45+CD3+CD4-CD8+, B-lymphocytes CD45+CD3-CD19+, NK-lymphocytes CD45+CD3-CD16+CD56+.
EFFECT: application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Taking into account the universality of the pathogenesis of the wound process and the existence of substances predictably affecting the nature of the immune response in the skin and certain functions of its constituent cells (including reparative), the development of a new biotechnological agent for healing skin wounds of different etiology is justified and relevant.
This determined the aim and objectives of this study. Read more...
Цельгель искусственно повышает концентрацию сигнальных молекул в ране, чтобы активировать механизмы репарации и ускорить естественные механизмы регенерации кожи, которые у человека с возрастом ослабевают. Покупайте в клинику, покупайте себе...
I don't remember any names. I don't know who these children have become. I don't know which of them is in school today, who is already working, who is raising younger brothers and sisters, and who has long been swept away by a road that we don't even know exists. But I remember their eyes.
I want to take a breath – we have done it! The expedition Live Parallel 2013 – Papua New Guinea where we walked many kilometers through the jungle, spent tens of kilometers inside the tarantas and hundreds of kilometers on board small planes flying on the same principle as a route-taxi, has come to an end without loss and above all with the result! But it could be otherwise… Well, let us not talk about sad things but about interesting ones, so… Jakarta was the first place to see.
