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Goltsov Sergey
I serve the art of medical doubt

Способ получения жизнеспособной гетерогенной популяции клеток кожи (патент на изобретение)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

Ещё в блоге:

В поисках причины зуда неясного генеза
В поисках причины зуда неясного генеза

Предыдущий разговор закончился на том, что морфологические изменения структуры кожи, изменяя его качественные характеристики, могут становиться стимулом к изменению психики пациента… читать далее

METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM
METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM

Application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.

Third book
Third book

Surprisingly, there are 14 years between these books. That's exactly how long Leo Tolstoy felt one cycle of human maturation constitutes. He had an almost intimate idea of ​​dividing life into periods—conditional periods in which, every fourteen years, a person reaches a new level of understanding.

If, putting aside excessive modesty (briefly and strictly for scientific purposes), we apply this logic to ourselves, a curious picture emerges.

Comparative evaluation of quality of hematopoietic stem cells of umbilical cord blood at various methods of cryopreservation
Comparative evaluation of quality of hematopoietic stem cells of umbilical cord blood at various methods of cryopreservation

Studied the proliferative potential and viability of hematopoietic stem cells (HSC) cord blood (PB), depending on the method of cryopreservation. Cryopreservation of samples was carried out in software Planer freezer and liquid nitrogen vapor. Found that, for cryopreservation of samples GSK PC software should be used freezer, since this method of preservation of the cells remain vi- able and functional completeness.