SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
The only one of the three places on the planet covered with snow on the equator is Mount Irian Jaya. Along with other mountains it seizes the Baliem valley around the perimeter while I’m seized with the feeling of being carried to the primeval world...
Application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Studied the proliferative potential and viability of hematopoietic stem cells (HSC) cord blood (PB), depending on the method of cryopreservation. Cryopreservation of samples was carried out in software Planer freezer and liquid nitrogen vapor. Found that, for cryopreservation of samples GSK PC software should be used freezer, since this method of preservation of the cells remain vi- able and functional completeness.
