SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
The history of dermatology remembers many attempts to transform human skin, a dense tissue, into a liquid. The search for a method to separate the skin's cellular substrate to obtain a suspension and study the phenotype of its constituent cells became the basis for a long-term research, resulting in a patented method for digitally assessing the subpopulation composition of skin cells - the skin cytoimmunogram... read the full article
All my life, it seemed to me that answers make a person's thinking bigger and at the same time sharper. But over time, I realized that answers are born only where they are preceded by good questions. Not to the textbook or to the authority, but to reality itself — to what we see, but often do not notice. It is often said that human life is short. I am becoming more and more convinced that it can be "lengthened" — not by the number of years I have lived, but by the density of observations. In more detail...
or immediately into the telegram channel from observation to understanding
