SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
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However...s ago, my parents traveled to the Caucasian Mineral Waters. The mountains, the narzans, the clear air, and the long walks... Apparently, this is where my story began. At that time, I was a rather microscopic creature with a tail, but I was probably quite fast. According to biology, the fertilizing speed of a sperm cell is around 25 meters per second. This means that I was swimming much faster than I am now. However...
Application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.
Taking into account the universality of the pathogenesis of the wound process and the existence of substances predictably affecting the nature of the immune response in the skin and certain functions of its constituent cells (including reparative), the development of a new biotechnological agent for healing skin wounds of different etiology is justified and relevant.
This determined the aim and objectives of this study. Read more...
