SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
Surprisingly, there are 14 years between these books. That's exactly how long Leo Tolstoy felt one cycle of human maturation constitutes. He had an almost intimate idea of dividing life into periods—conditional periods in which, every fourteen years, a person reaches a new level of understanding.
If, putting aside excessive modesty (briefly and strictly for scientific purposes), we apply this logic to ourselves, a curious picture emerges.
International Festival in Travel and Tourism named after N.N. Miklouho-Maclay "Russian traveler" was held in Orel.
My lecture "Diagnosis: The Traveler", planned in three parts, one for each day of the festival, was received with interest by world-famous travelers. But, as they say, tell God about your plans and he will laugh - and it happened. The third part, in my opinion the most important, because of the time-bound regulation was not brought to the listeners. However, in this there was a plus, as this is the best reason to continue the relationship outside the festival and breaking the boundaries, regardless of the distance to complete the scheduled. Let's do it, my friends!
Достигнув очередного этапа по маршруту Перу-Боливия, экспедиция Живая Параллель, всем составом ощутила высоту 3200 метров. Лопнувшие сосуды глаз, необъяснимая усталость, повышенная утомляемость, головная боль, ломота в суставах плюс желание сжаться до размера рюкзачка, который висит на спине товарища и, спрятавшись в нём, отдаться размышлениям, а не борьбе с одышкой.
Is there an aesthetically more significant area of the skin than the face? And that's not the only question. In approaches to rosacea – chose for its localization is a person, there are many. The first of them – what is the "mechanics" of pathology? Read more...
