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Goltsov Sergey
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Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

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METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM
METHOD FOR DETERMINING SUBPOPULATION COMPOSITION OF SKIN CELLS AND OBTAINING SKIN CYTOIMMUNOGRAM

Application of this method allows to make a quantitative and functional characteristic of all the skin constituent elements for use in diagnosis.

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