SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
Taking into account the universality of the pathogenesis of the wound process and the existence of substances predictably affecting the nature of the immune response in the skin and certain functions of its constituent cells (including reparative), the development of a new biotechnological agent for healing skin wounds of different etiology is justified and relevant.
This determined the aim and objectives of this study. Read more...
Information about the history of medical institutions Tyumen, which used to belong to the Tobolsk province, has been preserved a little. It is known that in 1741 there was a hospital in the city, which had 3 attendants. The hospital was located at the monastery. In the first half of the 19th century (1845), there was already one hospital in the city. There were 5 officials (doctors) and 7 lower attendants (orderlies) in the medical department. In 1859, the city hospital was opened, considered by contemporaries to be the best in Western Europe. Siberia, which has six hospital rooms, a laboratory, a pharmacy, and an anatomical theater... The whole story
Day in day, exactly 5 years, in Mikhail Sharomov's custom workshop, we created this unique custom motorcycle named Buffalo with Harley Davidson V-ROD engine. From idea and concept, design and implementation in every technical detail, through years of friendship, creation and inspired creativity to final assembly and registration. About that in my short film "Buffalo".
Later we may ask ourselves where we are going and why. This thought came to my mind under the sign “CANNIBALISM POLICE”… But it was too late, we had to go farther consoling ourselves with the thought that we were among the very few people in the whole world who had made some steps forward from the sign. Read more...
