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Goltsov Sergey
I serve the art of medical doubt

Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

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Let's go, then we'll start, or why I started hosting this channel
Let's go, then we'll start, or why I started hosting this channel

All my life, it seemed to me that answers make a person's thinking bigger and at the same time sharper. But over time, I realized that answers are born only where they are preceded by good questions. Not to the textbook or to the authority, but to reality itself — to what we see, but often do not notice. It is often said that human life is short. I am becoming more and more convinced that it can be "lengthened" — not by the number of years I have lived, but by the density of observations. In more detail...

or immediately into the telegram channel from observation to understanding
 

Conceptualization of human skin cell subpopulation states
Conceptualization of human skin cell subpopulation states

The results of several patented inventions investigate the dynamics of human skin cell subpopulation states. The immunological phenomena discovered thereby change not only the picture of skin conditions, but also the ideas about approaches to the diagnosis and treatment of skin diseases... read the article in full

Ten thousand hours and one reason to be proud
Ten thousand hours and one reason to be proud

On January 4, 2026, it will be exactly three years since I returned to the pool after a thirty-year hiatus...

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Third book
Third book

Surprisingly, there are 14 years between these books. That's exactly how long Leo Tolstoy felt one cycle of human maturation constitutes. He had an almost intimate idea of ​​dividing life into periods—conditional periods in which, every fourteen years, a person reaches a new level of understanding.

If, putting aside excessive modesty (briefly and strictly for scientific purposes), we apply this logic to ourselves, a curious picture emerges.