Main page → Blog → creatively → Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

METHOD FOR OBTAINING A LIVABLE HETEROGENEOUS POPULATION OF SKIN CELLS

Patent holder: Goltsov Sergey Viktorovich
Type: patent for an invention
Patent number: RU 2502999 C1
Patent office: Russia
Publication year: 2013
Application number: 2012131976/15
Registration date: 25.07.2012
Publication date: 27.12.2013

eLIBRARY ID: 37521805

INTERNATIONAL PATENT CLASSIFICATION:
G01N 33/48 Research or analysis of materials by special methods not included in groups 1/00 - 31/00 / biological materials, e.g. blood, urine; devices for counting and measuring blood cells (hemocytometers)
C12N 5/071 Undifferentiated human, animal, or plant cells, e.g. cell lines; tissues; cultivation or preservation thereof; nutrient media therefor / cells or tissues of animals / cells or tissues of vertebrates, e.g. human cells or tissues

Ещё в блоге:

in search of another self

Хочу выйти из себя, чтобы осмотреться

Искренне поговорили с Региной Бурмистровой.

creatively

CYTOIMMUNOGRAM OF THE SKIN: A NEW METHOD FOR OBJECTIVE EVALUATION OF THE SKIN CELL SUBPOPULATION PROFILE

The history of dermatology remembers many attempts to transform human skin, a dense tissue, into a liquid. The search for a method to separate the skin's cellular substrate to obtain a suspension and study the phenotype of its constituent cells became the basis for a long-term research, resulting in a patented method for digitally assessing the subpopulation composition of skin cells - the skin cytoimmunogram... read the full article

graphomania on the occasion

Living with cannibals has turned out to be possible… if there’s a will!

Looking into the eyes of this female leader of the Korowai tribe who are still considered to be cannibals, one may think there will be a more vivid experience during our life but the state of shock is still holding us hard! Read more…

jungle of dermatology

В поисках причины зуда неясного генеза

Предыдущий разговор закончился на том, что морфологические изменения структуры кожи, изменяя его качественные характеристики, могут становиться стимулом к изменению психики пациента… читать далее