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Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

METHOD FOR OBTAINING A LIVABLE HETEROGENEOUS POPULATION OF SKIN CELLS

Patent holder: Goltsov Sergey Viktorovich
Type: patent for an invention
Patent number: RU 2502999 C1
Patent office: Russia
Publication year: 2013
Application number: 2012131976/15
Registration date: 25.07.2012
Publication date: 27.12.2013

eLIBRARY ID: 37521805

INTERNATIONAL PATENT CLASSIFICATION:
G01N 33/48 Research or analysis of materials by special methods not included in groups 1/00 - 31/00 / biological materials, e.g. blood, urine; devices for counting and measuring blood cells (hemocytometers)
C12N 5/071 Undifferentiated human, animal, or plant cells, e.g. cell lines; tissues; cultivation or preservation thereof; nutrient media therefor / cells or tissues of animals / cells or tissues of vertebrates, e.g. human cells or tissues

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graphomania on the occasion

День знакомства

Несусветная дурь – дни рождения. А точнее, поздравления с ними. Кто это вообще придумал? читать далее

creatively

To be remembered and used

Skin and venereal diseases are among the oldest diseases of mankind that have accompanied humans at all stages of evolution. It is worth noting that the oldest example of medical literature, namely a clay tablet with a Sumerian text found in Nippur (in modern Iraq), tells about the treatment of skin diseases. After tens of thousands of years, I want to talk and write about skin and venereal diseases.

creatively

Nature-Friendly Strategy for Growing Innovation: the Placental Approach

The creation of fertile innovative ecological environment in the country is constrained by barriers of a conceptual nature. The article identifies four groups of such barriers. The authors propose a strategy for overcoming them based on a comparison of functions necessary for the innovative development of social practices and the natural mechanisms of a new life birth. This allows us to formulate a number of pragmatic recommendations for creating an innovative eco-environment for a wide range of industries. By analogy with natural dynamics of obstetrics, the approach is called placental

Keywords: Innovation, innovation infrastructure, acceleration, eco-environment, placenta, development.

graphomania on the occasion

Is the patient rather alive than dead?

Recently I have been asked this question.

This has made me ponder. As you know only dead people do not perspire… If this question has been asked, if it concerns somebody including me and if my feelings give rise to new thoughts then he is rather alive. From the perspective of the doctor stating the facts of the patients being discontent of the quality of the medical care provided he may be dead!

But as the woman cannot be just a little bit pregnant then a person cannot be half or partly alive or dead. Besides ...