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Способ получения жизнеспособной гетерогенной популяции клеток кожи (патент на изобретение)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

СПОСОБ ПОЛУЧЕНИЯ ЖИЗНЕСПОСОБНОЙ ГЕТЕРОГЕННОЙ ПОПУЛЯЦИИ КЛЕТОК КОЖИ

Патентообладатель: Гольцов Сергей Викторович
Тип: патент на изобретение
Номер патента: RU 2502999 C1
Патентное ведомство: Россия
Год публикации: 2013
Номер заявки: 2012131976/15
Дата регистрации: 25.07.2012
Дата публикации: 27.12.2013

eLIBRARY ID: 37521805

МЕЖДУНАРОДНАЯ ПАТЕНТНАЯ КЛАССИФИКАЦИЯ:
G01N 33/48 Исследование или анализ материалов особыми способами, не отнесенными к группам 1/00 — 31/00 / биологических материалов, например крови, мочи; приборы для подсчета и измерения клеток крови (гемоцитометры)
C12N 5/071 Недифференцированные клетки человека, животных или растений, например, клеточные линии; ткани; культивирование или сохранение их; питательные среды для них / клетки или ткани животных / клетки или ткани позвоночных, например клетки или ткани человека

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