SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
As a doctor, I repeatedly observed the development of a human embryo from several cells, witnessing the mystery of the appearance of life in the embryological laboratory. Also, as when a long time ago an organism emerged from the first cells, and then a person developed, now having made a population of the planet in seven billion three hundred million, life on the Earth also originated. But, one thing is birth, and the other is development. Read more...
Day in day, exactly 5 years, in Mikhail Sharomov's custom workshop, we created this unique custom motorcycle named Buffalo with Harley Davidson V-ROD engine. From idea and concept, design and implementation in every technical detail, through years of friendship, creation and inspired creativity to final assembly and registration. About that in my short film "Buffalo".
Few of my colleagues remember the glorious time when not only a comprehensive approach based on objective knowledge was used in the treatment of skin diseases and sexually transmitted infections, but also Immunostimulants were used, especially for the treatment of late forms of syphilis. Further...
The Olympus of style is still held by the magic of original ornaments, bright colors, animal prints, fantastic accessories and massive adornments.
