SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
Любой предмет можно увидеть, потрогать рукой, обозначить именем, наделить смыслом. Это легко! А как быть с умозрительными объектами? Например число пять, это что? Как его подержать в руке? Не в смысле пять яблок, а как число. А воображение - можно ли его потрогать? Это будет посложнее. Или вот, например, культура – это что? Как её увидеть? И, можно ли? А изучить ее закономерности сохранения или развития? Сложно!
As a doctor, I repeatedly observed the development of a human embryo from several cells, witnessing the mystery of the appearance of life in the embryological laboratory. Also, as when a long time ago an organism emerged from the first cells, and then a person developed, now having made a population of the planet in seven billion three hundred million, life on the Earth also originated. But, one thing is birth, and the other is development. Read more...
Quite early on, I realized that to truly help a large number of patients, I needed to go beyond the individual approach and create a methodology. I recall the words of Edith Piaf, who said, "I don't sing for everyone—I sing for everyone." And yet, she sang for everyone. So it was for me—there was that inspiration, that moment when the idea of phenotypic dermatology first arose. It was from this moment that the path to a systemic view of the skin began—not as a surface, but as an entire biological universe.
