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Method for obtaining a viable heterogeneous population of skin cells (patent for invention)

SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.

EFFECT: skin cell separation and viability preservation.

METHOD FOR OBTAINING A LIVABLE HETEROGENEOUS POPULATION OF SKIN CELLS

Patent holder: Goltsov Sergey Viktorovich
Type: patent for an invention
Patent number: RU 2502999 C1
Patent office: Russia
Publication year: 2013
Application number: 2012131976/15
Registration date: 25.07.2012
Publication date: 27.12.2013

eLIBRARY ID: 37521805

INTERNATIONAL PATENT CLASSIFICATION:
G01N 33/48 Research or analysis of materials by special methods not included in groups 1/00 - 31/00 / biological materials, e.g. blood, urine; devices for counting and measuring blood cells (hemocytometers)
C12N 5/071 Undifferentiated human, animal, or plant cells, e.g. cell lines; tissues; cultivation or preservation thereof; nutrient media therefor / cells or tissues of animals / cells or tissues of vertebrates, e.g. human cells or tissues

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