SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
The history of dermatology remembers many attempts to transform human skin, a dense tissue, into a liquid. The search for a method to separate the skin's cellular substrate to obtain a suspension and study the phenotype of its constituent cells became the basis for a long-term research, resulting in a patented method for digitally assessing the subpopulation composition of skin cells - the skin cytoimmunogram... read the full article
The theory of phenotypic dermatology: technologies and research that are changing the understanding of the diagnosis and treatment of skin diseases.
Quite early on, I realized that to truly help a large number of patients, I needed to go beyond the individual approach and create a methodology. I recall the words of Edith Piaf, who said, "I don't sing for everyone—I sing for everyone." And yet, she sang for everyone. So it was for me—there was that inspiration, that moment when the idea of phenotypic dermatology first arose. It was from this moment that the path to a systemic view of the skin began—not as a surface, but as an entire biological universe.
Studied the proliferative potential and viability of hematopoietic stem cells (HSC) cord blood (PB), depending on the method of cryopreservation. Cryopreservation of samples was carried out in software Planer freezer and liquid nitrogen vapor. Found that, for cryopreservation of samples GSK PC software should be used freezer, since this method of preservation of the cells remain vi- able and functional completeness.
