The other day I happened to freeze my hands. Yes, not that frostbite, and not frostbite at all, but so, slightly freeze. All right, all right-my gloved fingers froze, so I remembered a story told by a patient at the reception.
SUBSTANCE: invention provides a method for producing a heterogenous skin cell population implying the sampling of a skin biopsy material at a depth of 2 mm, tissue homogenisation in 0.9% aqueous sodium chloride at temperature +23 … +25°C, recovery of homogenate, filtration of the homogenate through an inert filter cloth with the pore diameter of 20 mcm, centrifugation of the homogenate at 400 g for 5 minutes at temperature +23 … +25 C.
EFFECT: skin cell separation and viability preservation.
