A device for activating the reparative potencies of skin cells (utility model patent)

The utility model relates to biotechnology and medicine, namely to devices for working with human tissue cells. The device contains containers made of opaque material in the form of truncated cones inserted into each other, which additionally have protruding annular collars with symmetrical chamfers on the side surface for sealing, and the upper truncated cone is filled with the active substance "Cellgel", the upper end of the upper truncated cone is closed with a flat blind lid, and its lower end It is a membrane with evenly distributed holes with a diameter of 5-7 microns., In this case, the lower truncated cone is filled with a divided viable heterogeneous population of skin cells in the form of a suspension. Sunlight is prevented from entering the device, limited contact of the active substance in the form of a gel with activated skin cells and their localization in a separate container is ensured.

The objectives of the claimed solution are to create a device that increases isolation from the external environment and the convenience of extracting activated skin cells, as well as allowing the use of gels as an active ingredient.

When implementing the utility model, the task is solved by achieving a technical result, which consists in ensuring limited contact of the active substance in the form of a gel with activated skin cells and their localization in a separate container with increased isolation from the external environment.

The specified technical result is achieved after activating the reparative potencies of skin cells in a device containing containers made of opaque material in the form of truncated cones inserted into each other, which additionally have protruding annular collars with symmetrical sealing chamfers on the side surface, and the upper truncated cone is filled with the active substance "Celgel", the upper end of the upper truncated cone is closed with a flat It has a blind lid, and its lower end is a membrane with evenly distributed holes with a diameter of 5-7 microns., In this case, the lower truncated cone is filled with a divided viable heterogeneous population of skin cells in the form of a suspension.

There is the following causal relationship between the claimed technical result and the essential features of the invention: increased isolation from the external environment is ensured by the manufacture of a device made of an opaque material that prevents the penetration of sunlight to the active substance and activated skin cells; increased convenience of extracting activated skin cells is ensured by their localization in a separate container – the lower truncated cone.; limited contact of the active substance in the form of a gel with activated skin cells occurs as a result of the placement of the active substance in the upper truncated cone, the biologically active molecules of which, due to the density gradient, diffuse through a membrane with a hole diameter of 5-7 microns to the activated skin cells located in the lower truncated cone. The diameter of the holes in the membrane is selected based on the size of the skin cells, which range from 8 microns to 15 microns, to prevent the penetration of skin cells from the lower truncated cone into the active substance. The active ingredient is "Celgel", which allows not only to cultivate skin cells, but also to activate them to produce biologically active substances that contribute to the emergence of reparative potencies of skin cells. Activated skin cells are a divided viable heterogeneous population of skin cells in the form of a suspension. To ensure tightness, the upper truncated cone is provided with a blind flat lid. The tightness of the connection of the upper truncated cone with the lower one is ensured by protruding annular collars on their side surface and due to the conical shape of the containers as a result of constant compression of the lower truncated cone to the upper one. At the same time, the collars have symmetrical chamfers to facilitate the connection of the truncated cones with each other. In addition, the tightness of the lower truncated cone is additionally ensured by a hydraulic seal formed by the active substance in the upper truncated cone, which prevents the possible penetration of anything from the environment into activated skin cells.

The figures show a general view of the device for activating the reparative potencies of skin cells.

The device consists of an upper truncated cone 1 having a collar 2 on the side surface of an annular cone protruding outward, and a collar 3 on the upper end of an annular cone protruding outward for fixing a blind flat lid 4. The lower end of the upper truncated cone is a membrane 5 with evenly distributed holes 6 with a diameter of 5-7 microns. The inner cavity of the upper truncated cone is filled with the active substance "Celgel" 7. 

The lower truncated cone 8 has an annular collar 9 at the upper end, protruding into the cone, and the lower end 10 is blind. The inner cavity of the lower truncated cone 8 is filled with a separated viable heterogeneous population of skin cells in the form of a suspension 11. The collars 2 and 9 have symmetrical chamfers to facilitate the connection of the truncated cones to each other. Part of the active substance "Celgel" 7 is immersed in a suspension 11 of a separated viable heterogeneous population of skin cells. 

All structural elements of the device are made of an opaque material that is chemically inert to the active substance "Celgel" and a separated viable heterogeneous population of skin cells, such as polypropylene.

The device works as follows.

Initially, the upper truncated cone 1 is filled with the active substance "Celgel" 7 and a blind flat lid 4 is fixed on the collar 3 for sealing. The lower truncated cone 8 is filled with a divided viable heterogeneous population of skin cells in the form of a suspension 11. The truncated cones are connected to each other by inserting the upper truncated cone 1 into the lower 8, until they are fixed with collars 2 and 9. 

After that, biologically active molecules from the active substance "Celgel" 7 begin to diffuse into a divided viable heterogeneous population of skin cells along a density gradient through holes 6 with a diameter of 5-7 microns in the membrane 5 of the upper truncated cone 1, activating the cells of the epidermis and dermis in suspension 11, which begin to produce biologically active substances. After the required activation time has elapsed, the collar 9 is bent, the truncated cones are separated and the activated skin cells are removed from the lower cone 8.

This increases the isolation of the device from the external environment and the convenience of extracting activated skin cells, as well as the possible use of gels as an active ingredient.